Anticorps Recombinant de lapin anti-MDH1

MDH1 Recombinant Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241887A1

N° de cat : 84576-4-PBS

Synonymes

MDHA, KAR, EC:1.1.1.96, EC:1.1.1.37, Aromatic alpha-keto acid reductase

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Galerie de données de validation

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  WB analysis using 84576-4-RR (same clone as 84576-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84576-4-RR (MDH1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84576-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 84576-4-RR (same clone as 84576-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 84576-4-RR (MDH1 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84576-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 84576-4-RR (same clone as 84576-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 84576-4-RR (MDH1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84576-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84576-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84576-4-RR against Human MDH1 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

84576-4-PBS cible MDH1 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: MDH1 Protéine recombinante Ag8744
• Nom complet: malate dehydrogenase 1, NAD (soluble)
• Masse moléculaire calculée: 334 aa, 36 kDa
• Poids moléculaire observé: 35-37 kDa
• Numéro d’acquisition GenBank: BC001484
• Symbole du gène: MDH1
• Identification du gène (NCBI): 4190
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MDH1 (Malate dehydrogenase, cytoplasmic) is also named as MDHA and belongs to the LDH/MDH superfamily and MDH type 2 family which catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. It can exsit as a dimer and the dimeric MDH1 is the mitochondrial isoenzyme, whereas the tetrameric MDH2 is the glycosomal isoenzyme.(PMID:10693743)

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