Anticorps Recombinant de lapin anti-MDM1

• Phare
• Validé par KD/KO

MDM1 Recombinant Antibody for WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

230425E10

N° de cat : 83141-2-PBS

Synonymes

230425E10, Nuclear protein MDM1

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Galerie de données de validation

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  WB analysis using 83141-2-RR (same clone as 83141-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83141-2-RR (MDM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83141-2-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 83141-2-RR (same clone as 83141-2-PBS)

  WB result of MDM1 antibody (83141-2-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MDM1 transfected HEK-293 cells. This data was developed using the same antibody clone with 83141-2-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP00166-1

  Sandwich ELISA standard curve of MP00166-1, Human MDM1 Recombinant Matched Antibody Pair - PBS only. 83141-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag10541. 83141-2-PBS was HRP conjugated as the detection antibody. Range: 46.8-3000 pg/mL.

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  Sample test of MP00166-1

  The mean MDM1 concentration was determined to be 1,326.4 pg/mL in Raji cell extract based on a 4.0 mg/mL extract load.

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  IHC staining of mouse testis using 83141-2-RR (same clone as 83141-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83141-2-RR (MDM1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83141-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00166-1

  Cytometric bead array standard curve of MP00166-1, MDM1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83141-3-PBS. Detection antibody: 83141-2-PBS. Standard: Ag10541. Range: 0.625-20 ng/mL.

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  Affinity and Kinetic Characterization of 83141-2-PBS

  Biolayer interferometry (BLl) kinetic assays of 83141-2-PBS against Human MDM1 were performed. The affinity constant is 3.50 nM.

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  Affinity and Kinetic Characterization of 83141-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83141-2-RR against Human MDM1 were performed. The affinity constant is 3.50 nM.

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Informations sur le produit

83141-2-PBS cible MDM1 dans les applications de WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: MDM1 Protéine recombinante Ag10541
• Nom complet: Mdm1 nuclear protein homolog (mouse)
• Masse moléculaire calculée: 81 kDa
• Poids moléculaire observé: 102 kDa
• Numéro d’acquisition GenBank: BC028355
• Symbole du gène: MDM1
• Identification du gène (NCBI): 56890
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Mdm1 was upregulated during ciliogenesis in multiciliated tracheal epithelial cells and localized to centrioles and cilia when transfected into NIH/3T3 cells. Mdm1 is a microtubule-binding protein that inhibits centrosome duplication when overexpressed. Proteomic analysis revealed that Mdm1 was also detected in the mouse photoreceptor sensory cilium complex and the outer segment (OS) of photoreceptor cells in the retina, suggesting that Mdm1 might be involved in the regulation of the functions and structure of the centrosome, as well as cilia. (PMID: 36171205)

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