Anticorps Recombinant de lapin anti-MDMX/MDM4

MDMX/MDM4 Recombinant Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241936D11

N° de cat : 84534-5-PBS

Synonymes

MDMX, MDM4, p53-binding protein Mdm4, Mdm2-like p53-binding protein, HDMX

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Galerie de données de validation

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  WB analysis using 84534-5-RR (same clone as 84534-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84534-5-RR (MDMX/MDM4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84534-5-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary cancer using 84534-5-RR (same clone as 84534-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84534-5-RR (MDMX/MDM4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84534-5-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 84534-5-RR (same clone as 84534-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84534-5-RR (MDMX/MDM4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84534-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84534-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84534-5-RR against Human MDMX/MDM4 were performed. The affinity constant is 0.247 nM.

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Informations sur le produit

84534-5-PBS cible MDMX/MDM4 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: MDMX/MDM4 Protéine recombinante Ag26678
• Nom complet: Mdm4 p53 binding protein homolog (mouse)
• Masse moléculaire calculée: 490 aa, 55 kDa
• Poids moléculaire observé: 70-76 kDa
• Numéro d’acquisition GenBank: BC067299
• Symbole du gène: MDMX/MDM4
• Identification du gène (NCBI): 4194
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MDM4 is also named as MDMX. It inhibits the activity of the tumor suppressor p53 by primarily cooperating with the p53 feedback regulator MDM2. MDM4 resides mostly in the cytoplasm, but can be recruited to the nucleus by MDM2.(PMID:16511572). The predicted mass of the protein is 54 kDa, while the observed mass is 70-80 kDa, a difference which stated is probably due to phosphorylation or other posttranslational modification(PMID:9226370, 28097652). It can be ubiquitinated and degraded by MDM2(PMID:16163388). It has 5 isoforms produced by alternative splicing.

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