Anticorps Recombinant de lapin anti-MDMX/MDM4

MDMX/MDM4 Recombinant Antibody for IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

241936A3

N° de cat : 84534-6-RR

Synonymes

MDM4, MDMX, 241936A3, HDMX, Mdm2-like p53-binding protein

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Galerie de données de validation

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  IF Staining of U2OS using 84534-6-RR

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MDMX/MDM4 antibody (84534-6-RR, Clone: 241936A3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of A549 using 84534-6-RR

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using MDMX/MDM4 antibody (84534-6-RR, Clone: 241936A3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 84534-6-RR

  Biolayer interferometry (BLl) kinetic assays of 84534-6-RR against Human MDMX/MDM4 were performed. The affinity constant is 2.24 nM.

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Applications testées

• Résultats positifs en IF/ICC: cellules A549, cellules U2OS

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84534-6-RR cible MDMX/MDM4 dans les applications de IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: MDMX/MDM4 Protéine recombinante Ag26678
• Nom complet: Mdm4 p53 binding protein homolog (mouse)
• Masse moléculaire calculée: 490 aa, 55 kDa
• Numéro d’acquisition GenBank: BC067299
• Symbole du gène: MDMX/MDM4
• Identification du gène (NCBI): 4194
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MDM4 is also named as MDMX. It inhibits the activity of the tumor suppressor p53 by primarily cooperating with the p53 feedback regulator MDM2. MDM4 resides mostly in the cytoplasm, but can be recruited to the nucleus by MDM2.(PMID:16511572). The predicted mass of the protein is 54 kDa, while the observed mass is 70-80 kDa, a difference which stated is probably due to phosphorylation or other posttranslational modification(PMID:9226370, 28097652). It can be ubiquitinated and degraded by MDM2(PMID:16163388). It has 5 isoforms produced by alternative splicing.

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