Anticorps Polyclonal de lapin anti-ME1

• Phare
• Validé par KD/KO

ME1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 16619-1-PBS

Synonymes

EC:1.1.1.40, Malic enzyme 1, NADP-dependent malic enzyme, NADP-ME

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Galerie de données de validation

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  WB analysis using 16619-1-AP (same clone as 16619-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16619-1-AP (ME1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16619-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse placenta using 16619-1-AP (same clone as 16619-1-PBS)

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 16619-1-AP (ME1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16619-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 16619-1-AP (same clone as 16619-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16619-1-AP (ME1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16619-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse liver using 16619-1-AP (same clone as 16619-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16619-1-AP (ME1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16619-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse liver using 16619-1-AP (same clone as 16619-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16619-1-AP (ME1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16619-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse liver using 16619-1-AP (same clone as 16619-1-PBS)

  IP result of anti-ME1 (IP:16619-1-AP, 4ug; Detection:16619-1-AP 1:2000) with mouse liver tissue lysate 2400 ug. This data was developed using the same antibody clone with 16619-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 16619-1-AP (same clone as 16619-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 16619-1-AP (ME1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 16619-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 16619-1-AP (same clone as 16619-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 16619-1-AP (ME1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 16619-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 16619-1-AP (same clone as 16619-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 16619-1-AP (ME1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16619-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 16619-1-AP (same clone as 16619-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 16619-1-AP (ME1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16619-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 16619-1-AP (same clone as 16619-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta using 16619-1-AP (ME1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16619-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 16619-1-AP (same clone as 16619-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta using 16619-1-AP (ME1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16619-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 16619-1-AP (same clone as 16619-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ME1 antibody (16619-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 16619-1-PBS in a different storage buffer formulation.

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Informations sur le produit

16619-1-PBS cible ME1 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ME1 Protéine recombinante Ag9916
• Nom complet: malic enzyme 1, NADP(+)-dependent, cytosolic
• Masse moléculaire calculée: 572 aa, 64 kDa
• Poids moléculaire observé: 55-64 kDa
• Numéro d’acquisition GenBank: BC025246
• Symbole du gène: ME1
• Identification du gène (NCBI): 4199
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ME1(NADP-dependent malic enzyme) belongs to the malic enzymes family. This malic enzyme catalyzes the reversible oxidative decarboxylation of malate and is a link between the glycolytic pathway and the citric acid cycle. The reaction is L-malate plus NADP(+) to form pyruvate, CO(2), and NADPH. The predicted protein contains 572 amino acids and has a calculated molecular mass of 64.1 kDa.

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