Anticorps Monoclonal anti-ME1

ME1 Monoclonal Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2G9G9

N° de cat : 68117-1-PBS

Synonymes

NADP-ME, NADP-dependent malic enzyme, Malic enzyme 1, EC:1.1.1.40, 2G9G9

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Galerie de données de validation

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  WB analysis using 68117-1-Ig (same clone as 68117-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68117-1-Ig (ME1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68117-1-PBS in a different storage buffer formulation.

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  WB analysis using 68117-1-Ig (same clone as 68117-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68117-1-Ig (ME1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68117-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 68117-1-Ig (same clone as 68117-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ME1 antibody (68117-1-Ig, Clone: 2G9G9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68117-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 68117-1-Ig (same clone as 68117-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.8 ug ME1 Monoclonal antibody (68117-1-Ig, Clone:2G9G9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68117-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68117-1-PBS cible ME1 dans les applications de WB, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ME1 Protéine recombinante Ag9916
• Nom complet: malic enzyme 1, NADP(+)-dependent, cytosolic
• Masse moléculaire calculée: 572 aa, 64 kDa
• Poids moléculaire observé: 55-64 kDa
• Numéro d’acquisition GenBank: BC025246
• Symbole du gène: ME1
• Identification du gène (NCBI): 4199
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ME1(NADP-dependent malic enzyme) belongs to the malic enzymes family. This malic enzyme catalyzes the reversible oxidative decarboxylation of malate and is a link between the glycolytic pathway and the citric acid cycle. The reaction is L-malate plus NADP(+) to form pyruvate, CO(2), and NADPH.

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