Anticorps Monoclonal anti-ME2

• Phare
• Validé par KD/KO

ME2 Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

6E6E2

N° de cat : 67457-1-PBS

Synonymes

Malic enzyme 2, ME2, NAD ME

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Galerie de données de validation

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  WB analysis using 67457-1-Ig (same clone as 67457-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67457-1-Ig (ME2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67457-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67457-1-Ig (same clone as 67457-1-PBS)

  WB result of ME2 antibody (67457-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ME2 transfected HeLa cells. This data was developed using the same antibody clone with 67457-1-PBS in a different storage buffer formulation.

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  WB analysis of human peripheral blood platelets using 67457-1-Ig (same clone as 67457-1-PBS)

  Human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 67457-1-Ig (ME2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67457-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 67457-1-Ig (same clone as 67457-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 67457-1-Ig (ME2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67457-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 67457-1-Ig (same clone as 67457-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67457-1-Ig (ME2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67457-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 67457-1-Ig (same clone as 67457-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67457-1-Ig (ME2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67457-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 67457-1-Ig (same clone as 67457-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67457-1-Ig (ME2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67457-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 67457-1-Ig (same clone as 67457-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67457-1-Ig (ME2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67457-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 67457-1-Ig (same clone as 67457-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67457-1-Ig (ME2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67457-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67457-1-PBS cible ME2 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ME2 Protéine recombinante Ag20741
• Nom complet: malic enzyme 2, NAD(+)-dependent, mitochondrial
• Masse moléculaire calculée: 65 kDa
• Poids moléculaire observé: 54 kDa, 65 kDa
• Numéro d’acquisition GenBank: BC000147
• Symbole du gène: ME2
• Identification du gène (NCBI): 4200
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ME2(malic enzyme 2) is also named as NAD-ME and belongs to the malic enzymes family. ME1, ME2 are important for NADPH production, lipogenesis and glutamine metabolism, but ME2 has a more profound effect. ME2 is a genome-coded mitochondrial enzyme that converts malate to pyruvate and is involved in neuronal synthesis of the neurotransmitter γ-aminobutyric acid (GABA)(PMID:15532013). This protein has 2 isoforms produced by alternative splicing with the MW of 65 kDa and 54 kDa.

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