Anticorps Recombinant de lapin anti-MECR

MECR Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242151C5

N° de cat : 84729-4-PBS

Synonymes

242151C5, 2-enoyl thioester reductase, EC:1.3.1.104, Enoyl-[acyl-carrier-protein] reductase, mitochondrial, Nrbf1

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Galerie de données de validation

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  WB analysis using 84729-4-RR (same clone as 84729-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84729-4-RR (MECR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84729-4-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 84729-4-RR (same clone as 84729-4-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 84729-4-RR (MECR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84729-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 84729-4-RR (same clone as 84729-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 84729-4-RR (MECR antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84729-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 84729-4-RR (same clone as 84729-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 84729-4-RR (MECR antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84729-4-PBS in a different storage buffer formulation.

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  IF Staining of NIH/3T3 using 84729-4-RR (same clone as 84729-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using MECR antibody (84729-4-RR, Clone: 242151C5 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84729-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84729-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84729-4-RR against Mouse MECR were performed. The affinity constant is below 1 pM.

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Informations sur le produit

84729-4-PBS cible MECR dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: MECR Protéine recombinante Ag0465
• Nom complet: mitochondrial trans-2-enoyl-CoA reductase
• Masse moléculaire calculée: 40 kDa
• Poids moléculaire observé: 37-40 kDa
• Numéro d’acquisition GenBank: BC003864
• Symbole du gène: MECR
• Identification du gène (NCBI): 26922
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Mitochondrial 2-enoyl-acyl-carrier protein reductase (MECR) is an enzyme in the mitochondrial fatty acid synthase (mtFAS) pathway. MECR is involved in the synthesis of lipoic acid and is required for mitochondrial respiratory competence. MECR is highly expressed in skeletal and heart muscles and exhibits lower expression in placenta, liver, kidney, and pancreas. It is weakly expressed or not expressed in the lung (PMID: 32440681, PMID: 37734847, PMID: 38296034).

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