Anticorps Polyclonal de lapin anti-MED12

• Phare
• Validé par KD/KO

MED12 Polyclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 20028-1-AP

Synonymes

ARC240, CAG repeat protein 45, CAGH45, FGS1, HOPA, KIAA0192, MED12, mediator complex subunit 12, OKS, OPA containing protein, OPA1, TNRC11, TRAP230

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Galerie de données de validation

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  WB analysis of mouse liver using 20028-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 20028-1-AP (MED12 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 20028-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 20028-1-AP (MED12 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 20028-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 20028-1-AP (MED12 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Informations sur le produit

20028-1-AP cible MED12 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: mediator complex subunit 12
• Masse moléculaire calculée: 243 kDa
• Poids moléculaire observé: 243 kDa
• Numéro d’acquisition GenBank: NM_005120
• Symbole du gène: MED12
• Identification du gène (NCBI): 9968
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Mediator of RNA polymerase II transcription subunit 12 (MED12), also named Activator-recruited cofactor 240 kDa component, Thyroid hormone receptor-associated protein complex 230 kDa component. MED12 is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This subunit may specifically regulate transcription of targets of the Wnt signaling pathway and SHH signaling pathway. Molecular weight of MED12 is about 240kd.This antibody is specific to MED12.

Publications

Species	Application	Title
mouse	WB	EMBO J; Fatty acid synthesis is critical for stem cell pluripotency via promoting mitochondrial fission.; Authors - Lihua Wang
human	IHC,IF	Mod Pathol; MED12 and HMGA2 mutations: two independent genetic events in uterine leiomyoma and leiomyosarcoma.; Authors - Elizabeth Bertsch
human,mouse	WB,IHC,IF	Genes (Basel); MED12 Regulates Smooth Muscle Cell Functions and Participates in the Development of Aortic Dissection.; Authors - Yingchao Zhou ; KD Validated
human	IHC	Pathol Res Pract; Cyclin-dependent kinase 8 is an independent prognosticator in uterine leiomyosarcoma.; Authors - Nobuko Yasutake