• Phare
  • Validé par KD/KO

Anticorps Polyclonal de lapin anti-MED12

MED12 Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 20028-1-AP

Synonymes

HOPA, FGS1, CAGH45, CAG repeat protein 45, ARC240



Applications testées

Résultats positifs en WBtissu hépatique de souris
Résultats positifs en IF/ICCcellules HepG2

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

20028-1-AP cible MED12 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Réactivité citéeHumain, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène Peptide
Nom complet mediator complex subunit 12
Masse moléculaire calculée 243 kDa
Poids moléculaire observé 243 kDa
Numéro d’acquisition GenBankNM_005120
Symbole du gène MED12
Identification du gène (NCBI) 9968
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS with 0.02% sodium azide and 50% glycerol
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Mediator of RNA polymerase II transcription subunit 12 (MED12), also named Activator-recruited cofactor 240 kDa component, Thyroid hormone receptor-associated protein complex 230 kDa component. MED12 is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This subunit may specifically regulate transcription of targets of the Wnt signaling pathway and SHH signaling pathway. Molecular weight of MED12 is about 240kd.This antibody is specific to MED12.

Protocole

Product Specific Protocols
WB protocol for MED12 antibody 20028-1-APDownload protocol
IF protocol for MED12 antibody 20028-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

EMBO J

Fatty acid synthesis is critical for stem cell pluripotency via promoting mitochondrial fission.

Authors - Lihua Wang
humanIHC,IF

Mod Pathol

MED12 and HMGA2 mutations: two independent genetic events in uterine leiomyoma and leiomyosarcoma.

Authors - Elizabeth Bertsch
human,mouseWB,IHC,IF

Genes (Basel)

MED12 Regulates Smooth Muscle Cell Functions and Participates in the Development of Aortic Dissection.

Authors - Yingchao Zhou
  • KD Validated
humanIHC

Pathol Res Pract

Cyclin-dependent kinase 8 is an independent prognosticator in uterine leiomyosarcoma.

Authors - Nobuko Yasutake
humanIF

Naunyn Schmiedebergs Arch Pharmacol

Loureirin B improves H/R-induced hepatic ischemia-reperfusion injury by downregulating ALOX5 to regulate mitochondrial homeostasis

Authors - Zhaolong Yu
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