Anticorps Recombinant de lapin anti-MED12L

MED12L Recombinant Antibody for IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240101C11

N° de cat : 83540-1-PBS

Synonymes

Thyroid hormone receptor-associated-like protein, PRO0314, Mediator of RNA polymerase II transcription subunit 12-like protein, Mediator complex subunit 12-like protein, KIAA1635

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Galerie de données de validation

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  IHC staining of mouse brain using 83540-1-RR (same clone as 83540-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83540-1-RR (MED12L antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83540-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 83540-1-RR (same clone as 83540-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83540-1-RR (MED12L antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83540-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 83540-1-RR (same clone as 83540-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MED12L antibody (83540-1-RR, Clone: 240101C11 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83540-1-PBS in a different storage buffer formulation.

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  FC experiment of U2OS using 83540-1-RR (same clone as 83540-1-PBS)

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug MED12L Recombinant antibody (83540-1-RR, Clone:240101C11) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83540-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 83540-1-RR (same clone as 83540-1-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug MED12L Recombinant antibody (83540-1-RR, Clone:240101C11) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83540-1-PBS in a different storage buffer formulation.

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Informations sur le produit

83540-1-PBS cible MED12L dans les applications de IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: MED12L Protéine recombinante Ag34636
• Nom complet: mediator complex subunit 12-like
• Masse moléculaire calculée: 240 kDa
• Numéro d’acquisition GenBank: NM_053002
• Symbole du gène: MED12L
• Identification du gène (NCBI): 116931
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MED12L, also named as KIAA1635, TNRC11L, TRALP and TRALPUSH, belongs to the Mediator complex subunit 12 family. MED12L may be a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. MED12L is a mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. MED12L is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This antibody can recognize all the isoform of MED12L. This antibody has no cross-reaction to MED12.

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