Anticorps Polyclonal de lapin anti-MED13-Specific

MED13-Specific Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

N° de cat : 20029-1-AP

Synonymes

ARC250, DRIP250, HSPC221, KIAA0593, MED13, MED13-Specific, mediator complex subunit 13, THRAP1, TRAP240

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Galerie de données de validation

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  WB analysis using 20029-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20029-1-AP (MED13-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

20029-1-AP cible MED13-Specific dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: mediator complex subunit 13
• Masse moléculaire calculée: 239 kDa
• Poids moléculaire observé: 240-250 kDa
• Numéro d’acquisition GenBank: NM_005121
• Symbole du gène: MED13
• Identification du gène (NCBI): 9969
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MED13, also named as ARC250, KIAA0593, THRAP1 and TRAP240, belongs to the Mediator complex subunit 13 family. MED13 is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This antibody is specific to MED13. It has no cross reaction to MED13L.

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