Anticorps Monoclonal anti-MED18

MED18 Monoclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

2D10G8

N° de cat : 60467-1-Ig

Synonymes

2D10G8, Mediator complex subunit 18, p28b

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Galerie de données de validation

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  WB analysis using 60467-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60467-1-Ig (MED18 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 60467-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using MED18 antibody (60467-1-Ig, Clone: 2D10G8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules LNCaP, cellules HEK-293, cellules HeLa, cellules HSC-T6, cellules Jurkat, cellules K-562, cellules MCF-7, cellules NIH/3T3, cellules U2OS
• Résultats positifs en IF/ICC: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

60467-1-Ig cible MED18 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: MED18 Protéine recombinante Ag7495
• Nom complet: mediator complex subunit 18
• Masse moléculaire calculée: 24 kDa
• Poids moléculaire observé: 24 kDa
• Numéro d’acquisition GenBank: BC002694
• Symbole du gène: MED18
• Identification du gène (NCBI): 54797
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MED18, also named as Mediator of RNA polymerase II transcription subunit 18, is a 208 amino acid protein, which belongs to the Mediator complex subunit 18 family. MED18 is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. MED18 as a mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. MED18 is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

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