Anticorps Polyclonal de lapin anti-MED20

• Phare
• Validé par KD/KO

MED20 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(3)

Avis (2)

N° de cat : 17598-1-AP

Synonymes

hTRFP, MED 20, Mediator of RNA polymerase II transcription subunit 20, TRF-proximal protein homolog

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Galerie de données de validation

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  WB analysis of K-562 using 17598-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 17598-1-AP (MED20 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17598-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17598-1-AP (MED20 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules K-562, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below

Informations sur le produit

17598-1-AP cible MED20 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MED20 Protéine recombinante Ag11598
• Nom complet: mediator complex subunit 20
• Masse moléculaire calculée: 212 aa, 23 kDa
• Poids moléculaire observé: 28 kDa
• Numéro d’acquisition GenBank: BC040950
• Symbole du gène: MED20
• Identification du gène (NCBI): 9477
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MED20(Mediator of RNA polymerase II transcription subunit 20) is also named as TRFP and belongs to the Mediator complex subunit 20 family. The deduced 209-amino acid protein shares 44% amino identity with Drosophila Trfp. TRFP had an apparent molecular mass of 28 kD by SDS-PAGE. It is identified TRFP as a component of an RNA polymerase II-SRB complex. The TRFP-containing complex could substitute for core RNA polymerase II in driving basal transcription from a minimal promoter.

Publications

Species	Application	Title
mouse	WB	Nat Commun; Surplus fatty acid synthesis increases oxidative stress in adipocytes and lnduces lipodystrophy; Authors - Li Weng
human	WB	J Virol; Adenovirus L-E1A activates transcription through mediator complex-dependent recruitment of the super elongation complex.; Authors - Vijayalingam S S
mouse	WB	Cell Rep; The Mediator subunit MED20 organizes the early adipogenic complex to promote development of adipose tissues and diet-induced obesity.; Authors - Wen-Shuai Tang ; KO Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Raphaël (Verified Customer) (11-12-2024)

I work on MED20 variants and your antibody againt MED20 is the best. I tried 5 differents antibodies and yours is the only one really working

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: fibroblastes

FH

siting (Verified Customer) (05-25-2021)

clear band

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: mouse embryo heart

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