Anticorps Polyclonal de lapin anti-MED29

MED29 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 33366-1-PBS

Synonymes

Intersex-like protein, IXL, Mediator complex subunit 29, Mediator of RNA polymerase II transcription subunit 29

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Galerie de données de validation

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  WB analysis using 33366-1-AP (same clone as 33366-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 33366-1-AP (MED29 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 33366-1-PBS in a different storage buffer formulation.

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  IP experiment of HT-29 using 33366-1-AP (same clone as 33366-1-PBS)

  IP result of anti-MED29 (IP:33366-1-AP, 4ug; Detection:33366-1-AP 1:800) with HT-29 cells lysate 1760 ug. This data was developed using the same antibody clone with 33366-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 33366-1-AP (same clone as 33366-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 33366-1-AP (MED29 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 33366-1-PBS in a different storage buffer formulation.

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Informations sur le produit

33366-1-PBS cible MED29 dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MED29 Protéine recombinante Ag38949
• Nom complet: mediator complex subunit 29
• Masse moléculaire calculée: 21kDa，200aa
• Poids moléculaire observé: 21-25 kDa
• Numéro d’acquisition GenBank: NM_017592.2
• Symbole du gène: MED29
• Identification du gène (NCBI): 55588
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Mediator of RNA polymerase II transcription subunit 29 (MED29) is also named as Mediator complex subunit 29 and Intersex-like protein (IXL). MED29 is one of the most highly conserved proteins across species and is widely expressed in humans both during embryonic development and in adult tissues (PMID: 15555573). MED29 was overexpressed in pancreatic cancer and promoted pancreatic cancer cell viability (PMID: 21225629, PMID: 17332321). MED29 facilitated the epithelial-mesenchymal transition process in OSCC, thereby promoting migration and invasion (PMID: 39462350).

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