Anticorps Polyclonal de lapin anti-MED31

MED31 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 16590-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of mouse testis using 16590-1-AP (same clone as 16590-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 16590-1-AP (MED31 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16590-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 16590-1-AP (same clone as 16590-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 16590-1-AP (MED31 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16590-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 16590-1-AP (same clone as 16590-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MED31 antibody (16590-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16590-1-PBS in a different storage buffer formulation.

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Informations sur le produit

16590-1-PBS cible MED31 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MED31 Protéine recombinante Ag9008
• Nom complet: mediator complex subunit 31
• Masse moléculaire calculée: 131 aa, 16 kDa
• Poids moléculaire observé: 16 kDa
• Numéro d’acquisition GenBank: BC012539
• Symbole du gène: MED31
• Identification du gène (NCBI): 51003
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MED31, also named as SOH1 or CGI-125, is a 131 amino acid protein, which belongs to the Mediator complex subunit 31 family. MED31 localizes in the nucleus. MED31 as a component of the Mediator complex, a coactivator is involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. MED31 functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery.

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