Anticorps Polyclonal de lapin anti-MED6

MED6 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(1)

Avis (2)

N° de cat : 15338-1-AP

Synonymes

ARC33, hMed6, MED6, mediator complex subunit 6, NY REN 28

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Galerie de données de validation

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  WB analysis using 15338-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15338-1-AP (MED6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 15338-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15338-1-AP (MED6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 15338-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 15338-1-AP (MED6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 15338-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15338-1-AP (MED6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HepG2, cellules Jurkat
• Résultats positifs en IHC: tissu de cancer de la prostate humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

15338-1-AP cible MED6 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MED6 Protéine recombinante Ag7541
• Nom complet: mediator complex subunit 6
• Masse moléculaire calculée: 28 kDa
• Poids moléculaire observé: 28-32 kDa
• Numéro d’acquisition GenBank: BC004106
• Symbole du gène: MED6
• Identification du gène (NCBI): 10001
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MED6 is a part of mediator complex. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Publications

Species	Application	Title
human,mouse	WB	Mol Cell; An IDR-dependent mechanism for nuclear receptor control of Mediator interaction with RNA polymerase II; Authors - Haiyan Zhao

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

anh (Verified Customer) (04-18-2022)

claer band on target molecular weight , but sometimes have some unspecific band on membrane other size

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse tissue

FH

SITING (Verified Customer) (05-25-2021)

good

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: EMBRYO HEART

MED6 Antibody Western Blot validation (1:500 dilution) in EMBRYO HEART (Cat no:15338-1-AP)

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