Anticorps Polyclonal de lapin anti-MED6

MED6 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

N° de cat : 15338-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 15338-1-AP (same clone as 15338-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15338-1-AP (MED6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15338-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 15338-1-PBS

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15338-1-AP (MED6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 15338-1-AP (same clone as 15338-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 15338-1-AP (MED6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15338-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 15338-1-AP (same clone as 15338-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15338-1-AP (MED6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15338-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 15338-1-AP (same clone as 15338-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15338-1-AP (MED6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15338-1-PBS in a different storage buffer formulation.

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Informations sur le produit

15338-1-PBS cible MED6 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MED6 Protéine recombinante Ag7541
• Nom complet: mediator complex subunit 6
• Masse moléculaire calculée: 28 kDa
• Poids moléculaire observé: 28-32 kDa
• Numéro d’acquisition GenBank: BC004106
• Symbole du gène: MED6
• Identification du gène (NCBI): 10001
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MED6 is a part of mediator complex. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

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