Anticorps Recombinant de lapin anti-MED6

MED6 Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

241382H2

N° de cat : 84184-4-PBS

Synonymes

241382H2, Activator-recruited cofactor 33 kDa component, ARC33, hMed6, Mediator of RNA polymerase II transcription subunit 6

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Galerie de données de validation

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  WB analysis using 84184-4-RR (same clone as 84184-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84184-4-RR (MED6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84184-4-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01087-3

  Sandwich ELISA standard curve of MP01087-3, Human MED6 Recombinant Matched Antibody Pair - PBS only. 84184-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag7541. 84184-2-PBS was HRP conjugated as the detection antibody. Range: 156-5000 pg/mL

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  Sample test of MP01087-3

  The mean MED6 concentration was determined to be 7.45 ng/mL in HEK-293 cell extract based on a 1.5 mg/mL extract load, 16.00 ng/mL in Jurkat cell extract based on a 1.5 mg/mL extract load and 13.01 ng/mL in U2OS cell extract based on a 3.1 mg/mL extract load.

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  IF Staining of A431 using 84184-4-RR (same clone as 84184-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using MED6 antibody (84184-4-RR, Clone: 241382H2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84184-4-PBS in a different storage buffer formulation.

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  FC experiment of A431 using 84184-4-RR (same clone as 84184-4-PBS)

  1x10^6 A431 cells were intracellularly stained with 0.25 ug MED6 Recombinant antibody (84184-4-RR, Clone:241382H2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84184-4-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01087-3

  Cytometric bead array standard curve of MP01087-3, MED6 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84184-4-PBS. Detection antibody: 84184-2-PBS. Standard: Ag7541. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 84184-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84184-4-RR against Human MED6 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

84184-4-PBS cible MED6 dans les applications de WB, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: MED6 Protéine recombinante Ag7541
• Nom complet: mediator complex subunit 6
• Masse moléculaire calculée: 28 kDa
• Poids moléculaire observé: 33 kDa
• Numéro d’acquisition GenBank: BC004106
• Symbole du gène: MED6
• Identification du gène (NCBI): 10001
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MED6 is a part of mediator complex. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

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