Anticorps Recombinant de lapin anti-MED6

MED6 Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

241382H2

N° de cat : 84184-4-RR

Synonymes

241382H2, Activator-recruited cofactor 33 kDa component, ARC33, hMed6, Mediator of RNA polymerase II transcription subunit 6

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Galerie de données de validation

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  WB analysis using 84184-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84184-4-RR (MED6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 84184-4-RR

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using MED6 antibody (84184-4-RR, Clone: 241382H2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of A431 using 84184-4-RR

  1x10^6 A431 cells were intracellularly stained with 0.25 ug MED6 Recombinant antibody (84184-4-RR, Clone:241382H2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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  Affinity and Kinetic Characterization of 84184-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84184-4-RR against Human MED6 were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A431, cellules HepG2, cellules Jurkat, tissu cérébral de souris, tissu de côlon de souris
• Résultats positifs en IF/ICC: cellules A431,
• Résultats positifs en FC (Intra): cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84184-4-RR cible MED6 dans les applications de WB, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: MED6 Protéine recombinante Ag7541
• Nom complet: mediator complex subunit 6
• Masse moléculaire calculée: 28 kDa
• Poids moléculaire observé: 33 kDa
• Numéro d’acquisition GenBank: BC004106
• Symbole du gène: MED6
• Identification du gène (NCBI): 10001
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MED6 is a part of mediator complex. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

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