Anticorps Polyclonal de lapin anti-MED8

MED8 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 12182-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of HepG2 using 12182-1-AP (same clone as 12182-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12182-1-AP (MED8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12182-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 12182-1-AP (same clone as 12182-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12182-1-AP (MED8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12182-1-PBS in a different storage buffer formulation.

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  WB analysis of SGC-7901 using 12182-1-AP (same clone as 12182-1-PBS)

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 12182-1-AP (MED8 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12182-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 12182-1-AP (same clone as 12182-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12182-1-AP (MED8 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12182-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 12182-1-AP (same clone as 12182-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12182-1-AP (MED8 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12182-1-PBS in a different storage buffer formulation.

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Informations sur le produit

12182-1-PBS cible MED8 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MED8 Protéine recombinante Ag2827
• Nom complet: mediator complex subunit 8
• Masse moléculaire calculée: 212 aa, 23 kDa
• Poids moléculaire observé: 29 kDa
• Numéro d’acquisition GenBank: BC010543
• Symbole du gène: MED8
• Identification du gène (NCBI): 112950
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MED8, a component of the Mediator complex, is a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. It plays a role as a target recruitment subunit in E3 ubiquitin-protein ligase complexes and thus in ubiquitination and subsequent proteasomal degradation of target proteins. MED8 has three isoforms with MW 19 kDa, 29 kDa and 33 kDa. And the ubiquitination form will be 38-43 kDa.

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