Anticorps Recombinant de lapin anti-MEF2D

MEF2D Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250068B7

N° de cat : 85801-5-PBS

Synonymes

Myocyte-specific enhancer factor 2D

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Galerie de données de validation

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  WB analysis using 85801-5-RR (same clone as 85801-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85801-5-RR (MEF2D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85801-5-PBS in a different storage buffer formulation.

• 

  WB analysis using 85801-5-RR (same clone as 85801-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85801-5-RR (MEF2D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85801-5-PBS in a different storage buffer formulation.

• 

  WB analysis using 85801-5-RR (same clone as 85801-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85801-5-RR (MEF2D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85801-5-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 85801-5-RR (same clone as 85801-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85801-5-RR (MEF2D antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85801-5-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 85801-5-RR (same clone as 85801-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85801-5-RR (MEF2D antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85801-5-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 85801-5-RR (same clone as 85801-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MEF2D antibody (85801-5-RR, Clone: 250068B7 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85801-5-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 85801-5-RR

  Biolayer interferometry (BLl) kinetic assays of 85801-5-RR against Human MEF2D were performed. The affinity constant is 1.99 nM.

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Informations sur le produit

85801-5-PBS cible MEF2D dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: MEF2D Protéine recombinante Ag5700
• Nom complet: myocyte enhancer factor 2D
• Masse moléculaire calculée: 56 kDa
• Poids moléculaire observé: 55-60 kDa
• Numéro d’acquisition GenBank: BC040949
• Symbole du gène: MEF2D
• Identification du gène (NCBI): 4209
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MEF2D, also named as Myocyte-specific enhancer factor 2D, is a 521 amino acid protein, which belongs to the MEF2 family. MEF2D is expressed in t in myotubes and also in undifferentiated myoblasts. MEF2D as a transcriptional activator which binds specifically to the MEF2 element, found in numerous muscle-specific and stress-induced genes. MEF2D mediates cellular functions not only in skeletal and cardiac muscle development, but also in neuronal differentiation and survival. The calculated molecular weight of MEF2D is 55 kDa, but Phosphorylated MEF2D is 60 kDa.

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