Anticorps Monoclonal anti-MEK1

• Phare
• Validé par KD/KO

MEK1 Monoclonal Antibody for WB, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3F6D7

N° de cat : 67872-1-PBS

Synonymes

MAP2K1, MAPKK 1, MAP2K 1, EC:2.7.12.2, Dual specificity mitogen-activated protein kinase kinase 1

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Galerie de données de validation

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  WB analysis of A431 using 67872-1-Ig (same clone as 67872-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 67872-1-Ig (MEK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67872-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67872-1-Ig (same clone as 67872-1-PBS)

  Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 67872-1-Ig (Phospho-MEK1 (Thr292) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67872-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67872-1-Ig (same clone as 67872-1-PBS)

  WB result of MEK1 antibody (67872-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MEK1 transfected HeLa cells. This data was developed using the same antibody clone with 67872-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 67872-1-Ig (same clone as 67872-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67872-1-Ig (Phospho-MEK1 (Thr292) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67872-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 67872-1-Ig (same clone as 67872-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67872-1-Ig (Phospho-MEK1 (Thr292) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67872-1-PBS in a different storage buffer formulation.

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  WB analysis of PC-3 using 67872-1-Ig (same clone as 67872-1-PBS)

  Calyculin A treated PC-3 cells were subjected to SDS PAGE followed by western blot with 67872-1-Ig (Phospho-MEK1 (Thr292) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67872-1-PBS in a different storage buffer formulation.

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  Cytometric bead array in cell lysate using MP50180-1

  Cytometric bead array in cell lysate using MP50180-1, Phospho-MEK1 (Thr386) Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67872-1-PBS. Detection antibody: 68015-1-PBS. Cell lysate: Non-treated HeLa and Calyculin A treated HeLa (30μg/well). Non-related target OAT Monoclonal Matched Antibody Pair (MP50109-1P) was served as control.

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Informations sur le produit

67872-1-PBS cible MEK1 dans les applications de WB, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: mitogen-activated protein kinase kinase 1
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 40-50 kDa
• Numéro d’acquisition GenBank: BC139729
• Symbole du gène: MEK1
• Identification du gène (NCBI): 5604
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MEK1 is also named as MAP2K1 (mitogen-activated protein kinase kinase 1), MAPKK1, PRKMK1, MKK1 and belongs to the MAP kinase kinase subfamily. It is a dual-specificity kinase that mediate ERK1 and ERK2 activation during adhesion and growth factor signaling(PMID:19219045). It also plays an essential role in extra-embryonic ectoderm during placentogenesis.

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