Anticorps Monoclonal anti-MEK1/2

MEK1/2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1H8C2

N° de cat : 67410-1-PBS

Synonymes

MAP2K2, MEK2, MAP kinase kinase 2, ERK activator kinase 2, EC:2.7.12.2

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Galerie de données de validation

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  WB analysis using 67410-1-Ig (same clone as 67410-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67410-1-Ig (MEK1/2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67410-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67410-1-Ig (same clone as 67410-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67410-1-Ig (MEK1/2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67410-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67410-1-Ig (same clone as 67410-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67410-1-Ig (MEK1/2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67410-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67410-1-Ig (same clone as 67410-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67410-1-Ig (MEK1/2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67410-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67410-1-Ig (same clone as 67410-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67410-1-Ig (MEK1/2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67410-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67410-1-Ig (same clone as 67410-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MEK1/2 antibody (67410-1-Ig, Clone: 1H8C2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67410-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67410-1-PBS cible MEK1/2 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: MEK1/2 Protéine recombinante Ag29871
• Nom complet: mitogen-activated protein kinase kinase 2
• Masse moléculaire calculée: 44 kDa
• Poids moléculaire observé: 44-47 kDa
• Numéro d’acquisition GenBank: BC018645
• Symbole du gène: MEK2
• Identification du gène (NCBI): 5605
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MEK2(MAPK/ERK kinase 2) is also named as MAP2K2, MKK2, PRKMK2 and belongs to the MAP kinase kinase subfamily. MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1). MEK1 and MEK2 are closely related, dual-specificity tyrosine/threonine protein kinases found in the Ras/Raf/MEK/ERK mitogen-activated protein kinase (MAPK) signaling pathway. 67410-1-Ig can recognize both the MEK2 and MEK1.

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