Anticorps Polyclonal de lapin anti-MEKK3

MEKK3 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 21072-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of mouse liver using 21072-1-AP (same clone as 21072-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 21072-1-AP (MEKK3 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21072-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse liver using 21072-1-AP (same clone as 21072-1-PBS)

  IP result of anti-MEKK3 (IP:21072-1-AP, 4ug; Detection:21072-1-AP 1:300) with mouse liver tissue lysate 7000ug. This data was developed using the same antibody clone with 21072-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 21072-1-AP (same clone as 21072-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21072-1-AP (MEKK3 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21072-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 21072-1-AP (same clone as 21072-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21072-1-AP (MEKK3 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21072-1-PBS in a different storage buffer formulation.

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Informations sur le produit

21072-1-PBS cible MEKK3 dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MEKK3 Protéine recombinante Ag15223
• Nom complet: mitogen-activated protein kinase kinase kinase 3
• Masse moléculaire calculée: 657 aa, 74 kDa
• Poids moléculaire observé: 71 kDa, 74 kDa
• Numéro d’acquisition GenBank: BC090859
• Symbole du gène: MEKK3
• Identification du gène (NCBI): 4215
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The mitogen-activated protein/ERK kinase kinase 3 (MEKK3) is a member of a family of MEKK/Ste11 serine/threonine protein kinases. One function of MEKK3 is to serve as an upstream regulatory kinase in the mitogen-activated protein kinase cascade, however, it also plays a critical role in other signaling pathways. MEKK3 regulates several important functions including cell survival and proliferation. It is ubiquitously expressed in multiple tissues and has been found in complexes with scaffold proteins and other kinases. (PMID: 18206350).Cerebral cavernous malformations (CCMs) complex regulation of MEKK3 is essential during vertebrate heart development. MEKK3 signalling and KLF2/4 may be targeted to develop new CCM therapeutics.

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