Anticorps Recombinant de lapin anti-MELK

MELK Recombinant Antibody for FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

230106D4

N° de cat : 82893-1-PBS

Synonymes

KIAA0175, hPK38, hMELK, EC:2.7.11.1, EC:2.7.10.2

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Galerie de données de validation

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  FC experiment of MCF-7 using 82893-1-RR (same clone as 82893-1-PBS)

  1x10^6 MCF-7 cells were intracellularly stained with 0.5 ug Anti-Human MELK (82893-1-RR, Clone:230106D4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:166 (red), or 0.5 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82893-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82893-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82893-1-RR against Human MELK were performed. The affinity constant is below 1 pM.

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Informations sur le produit

82893-1-PBS cible MELK dans les applications de FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: MELK Protéine recombinante Ag1897
• Nom complet: maternal embryonic leucine zipper kinase
• Masse moléculaire calculée: 651 aa, 75 kDa
• Numéro d’acquisition GenBank: BC014039
• Symbole du gène: MELK
• Identification du gène (NCBI): 9833
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MELK is involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. It also plays a key role in cell proliferation and carcinogenesis, and it is required for proliferation of embryonic and postnatal multipotent neural progenitors.

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