Anticorps Recombinant de lapin anti-MELK

• Phare
• Validé par KD/KO

MELK Recombinant Antibody for WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

230106A4

N° de cat : 82893-2-PBS

Synonymes

230106A4, EC:2.7.10.2, EC:2.7.11.1, hMELK, hPK38

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Galerie de données de validation

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  WB analysis using 82893-2-RR (same clone as 82893-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82893-2-RR (MELK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82893-2-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 82893-2-RR (same clone as 82893-2-PBS)

  WB result of MELK antibody (82893-2-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MELK transfected HeLa cells. This data was developed using the same antibody clone with 82893-2-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP00075-4

  Sandwich ELISA standard curve of MP00075-4, Human MELK Recombinant Matched Antibody Pair - PBS only. 82893-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag1897. 82893-6-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL

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  Sample test of MP00075-4

  NCCIT and K-562 cell lysate were measured. The human MELK concentration of detected samples was determined to be 15.9 ng/mL (based on a 4.5 mg/mL extract load) in NCCIT cell lysate and 16.4 ng/mL (based on a 4.5 mg/mL extract load) in K-562 cell lysate.

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  IHC staining of mouse kidney using 82893-2-RR (same clone as 82893-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 82893-2-RR (MELK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82893-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 82893-2-RR (same clone as 82893-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 82893-2-RR (MELK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82893-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82893-2-RR

  Biolayer interferometry (BLl) kinetic assays of 82893-2-RR against Human MELK were performed. The affinity constant is below 1 pM.

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Informations sur le produit

82893-2-PBS cible MELK dans les applications de WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: MELK Protéine recombinante Ag1897
• Nom complet: maternal embryonic leucine zipper kinase
• Masse moléculaire calculée: 651 aa, 75 kDa
• Poids moléculaire observé: 50-70 kDa
• Numéro d’acquisition GenBank: BC014039
• Symbole du gène: MELK
• Identification du gène (NCBI): 9833
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MELK is involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. It also plays a key role in cell proliferation and carcinogenesis, and it is required for proliferation of embryonic and postnatal multipotent neural progenitors.

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