Anticorps Polyclonal de lapin anti-MERTK

MERTK Polyclonal Antibody for WB, IHC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 27900-1-AP

Synonymes

c mer, EC:2.7.10.1, MER, Proto oncogene c Mer, Proto-oncogene c-Mer

---

Galerie de données de validation

• 

  WB analysis using 27900-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27900-1-AP (MERTK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of rat liver using 27900-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 27900-1-AP (MERTK antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 27900-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 27900-1-AP (MERTK antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 27900-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 27900-1-AP (MERTK antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of Jurkat using 27900-1-AP

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug MERTK Polyclonal antibody (27900-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules U-937, tissu testiculaire de souris
• Résultats positifs en IHC: tissu hépatique de rat, tissu pulmonaire de souris, tissu pulmonaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en FC (Intra): cellules Jurkat,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

27900-1-AP cible MERTK dans les applications de WB, IHC, IF, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MERTK Protéine recombinante Ag27540
• Nom complet: c-mer proto-oncogene tyrosine kinase
• Masse moléculaire calculée: 999 aa, 110 kDa
• Poids moléculaire observé: 160-180 kDa
• Numéro d’acquisition GenBank: BC114918
• Symbole du gène: MERTK
• Identification du gène (NCBI): 10461
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MerTK (Mer tyrosine kinase), also known as RP38, c-Eyk, c-mer, and Tyro12, was first cloned from a human B lymphoblastoid expression library (PMID:8086340) and is one of the TAM (Tyro-3, Axl, and MerTK) receptor tyrosine kinase (RTK) family (PMID:23833304). Although this RTK, like others, can promote tumor cell proliferation to some extent, MERTK primarily lends tumor cells crucial survival advantages while promoting invasion, migration and metastasis, drug resistance, and, in the innate immune system, suppressing anti-tumor immunity (PMID:32417270). The multiple MERTK species observed are likely due to posttranslational modifications (PMID: 23585477).

Publications

Species	Application	Title
human	IF	Cell Rep Med; Disruption of MerTK increases the efficacy of checkpoint inhibitor by enhancing ferroptosis and immune response in hepatocellular carcinoma; Authors - Shun Wang
human	IHC	Eur Arch Otorhinolaryngol; Aberrant expressions of TAM receptors are associated with postoperative recurrence in chronic rhinosinusitis with nasal polyps; Authors - Yandan Zhou
human	WB	Front Endocrinol (Lausanne); Downregulation of MerTK in circulating T cells of patients with non-proliferative diabetic retinopathy; Authors - Shimiao Bu
mouse	WB	J Ethnopharmacol; Longan Aril polysaccharides ameliorate cognitive impairment in AD mice via restoration of the immune phagocytosis of microglia; Authors - Han Zhang
mouse	WB	J Ethnopharmacol; Xin-shu-bao tablets ameliorates ventricular remodeling against HFrEF via PPARγ/MFGE8 pathway based on MALDI-MSI and Lipidomics; Authors - Fengrong Zhang

{{ptg:RelatedPrimaryAntibodies}}