Anticorps Polyclonal de lapin anti-MERTK

MERTK Polyclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 27900-1-PBS

Synonymes

c mer, EC:2.7.10.1, MER, Proto oncogene c Mer, Proto-oncogene c-Mer

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Galerie de données de validation

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  WB analysis using 27900-1-AP (same clone as 27900-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 27900-1-AP (MERTK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27900-1-PBS in a different storage buffer formulation.

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  IHC staining of rat liver using 27900-1-AP (same clone as 27900-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 27900-1-AP (MERTK antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27900-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung using 27900-1-AP (same clone as 27900-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 27900-1-AP (MERTK antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27900-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse lung using 27900-1-AP (same clone as 27900-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 27900-1-AP (MERTK antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27900-1-PBS in a different storage buffer formulation.

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  FC experiment of Jurkat using 27900-1-AP (same clone as 27900-1-PBS)

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug MERTK Polyclonal antibody (27900-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 27900-1-PBS in a different storage buffer formulation.

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Informations sur le produit

27900-1-PBS cible MERTK dans les applications de WB, IHC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MERTK Protéine recombinante Ag27540
• Nom complet: c-mer proto-oncogene tyrosine kinase
• Masse moléculaire calculée: 999 aa, 110 kDa
• Poids moléculaire observé: 160-180 kDa
• Numéro d’acquisition GenBank: BC114918
• Symbole du gène: MERTK
• Identification du gène (NCBI): 10461
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MerTK (Mer tyrosine kinase), also known as RP38, c-Eyk, c-mer, and Tyro12, was first cloned from a human B lymphoblastoid expression library (PMID:8086340) and is one of the TAM (Tyro-3, Axl, and MerTK) receptor tyrosine kinase (RTK) family (PMID:23833304). Although this RTK, like others, can promote tumor cell proliferation to some extent, MERTK primarily lends tumor cells crucial survival advantages while promoting invasion, migration and metastasis, drug resistance, and, in the innate immune system, suppressing anti-tumor immunity (PMID:32417270). The multiple MERTK species observed are likely due to posttranslational modifications (PMID: 23585477).

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