Anticorps Recombinant de lapin anti-MERTK

MERTK Recombinant Antibody for WB, IHC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

241252H3

N° de cat : 84156-2-PBS

Synonymes

241252H3, c mer, EC:2.7.10.1, MER, Proto oncogene c Mer

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Galerie de données de validation

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  WB analysis using 84156-2-RR (same clone as 84156-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84156-2-RR (MERTK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84156-2-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01061-2

  Sandwich ELISA standard curve of MP01061-2, Human MERTK Recombinant Matched Antibody Pair - PBS only. 84156-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag27540. 84156-1-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

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  Sample test of MP01061-2

  The mean MERTK concentration was determined to be 5,583.9 pg/mL in HepG2 cell extract based on a 2.3 mg/mL extract load.

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  IHC staining of mouse kidney using 84156-2-RR (same clone as 84156-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84156-2-RR (MERTK antibody) at dilution of 1:250 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84156-2-PBS in a different storage buffer formulation.

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  FC experiment of Jurkat using 84156-2-RR (same clone as 84156-2-PBS)

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug MERTK Recombinant antibody (84156-2-RR, Clone:241252H3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84156-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84156-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84156-2-RR against Human MERTK were performed. The affinity constant is 0.444 nM.

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Informations sur le produit

84156-2-PBS cible MERTK dans les applications de WB, IHC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: MERTK Protéine recombinante Ag27540
• Nom complet: c-mer proto-oncogene tyrosine kinase
• Masse moléculaire calculée: 999 aa, 110 kDa
• Poids moléculaire observé: 160-180 kDa
• Numéro d’acquisition GenBank: BC114918
• Symbole du gène: MERTK
• Identification du gène (NCBI): 10461
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MerTK (Mer tyrosine kinase), also known as RP38, c-Eyk, c-mer, and Tyro12, was first cloned from a human B lymphoblastoid expression library (PMID:8086340) and is one of the TAM (Tyro-3, Axl, and MerTK) receptor tyrosine kinase (RTK) family (PMID:23833304). Although this RTK, like others, can promote tumor cell proliferation to some extent, MERTK primarily lends tumor cells crucial survival advantages while promoting invasion, migration and metastasis, drug resistance, and, in the innate immune system, suppressing anti-tumor immunity (PMID:32417270). The multiple MERTK species observed are likely due to posttranslational modifications (PMID: 23585477).

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