Anticorps Polyclonal de lapin anti-MEST

• Phare
• Validé par KD/KO

MEST Polyclonal Antibody for IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

12

N° de cat : 11118-1-AP

Synonymes

MEST, PEG1

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Galerie de données de validation

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  WB analysis of human testis using 11118-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 11118-1-AP (MEST antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 11118-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 11118-1-AP (MEST antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 11118-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11118-1-AP (MEST antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 11118-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11118-1-AP (MEST antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 11118-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 11118-1-AP (MEST antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 11118-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 11118-1-AP (MEST antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 11118-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 11118-1-AP (MEST antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of PC-3 using 11118-1-AP

  IP Result of anti-MEST (IP:11118-1-AP, 3ug; Detection:11118-1-AP 1:500) with PC-3 cells lysate 1720ug.

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  IHC staining of human pancreas cancer using 11118-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11118-1-AP (MEST antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 11118-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11118-1-AP (MEST antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu testiculaire humain, cellules PC-3, tissu testiculaire de souris
• Résultats positifs en IP: cellules PC-3
• Résultats positifs en IHC: tissu de cancer du pancréas humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IHC: See 3 publications below
• IF: See 3 publications below

Informations sur le produit

11118-1-AP cible MEST dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MEST Protéine recombinante Ag1617
• Nom complet: mesoderm specific transcript homolog (mouse)
• Masse moléculaire calculée: 38 kDa
• Poids moléculaire observé: 34 kDa
• Numéro d’acquisition GenBank: BC011908
• Symbole du gène: MEST
• Identification du gène (NCBI): 4232
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MEST(Mesoderm-specific transcript homolog protein) is also named as PEG1 and belongs to the AB hydrolase superfamily. The gene is a imprinting gene, which is associated with growth of mesodermal origin cells(PMID:20339302) and plays important roles in embryo development(PMID:21375506). It is also a novel regulator of Wnt/CTNNB signalling during adipogenic differentiation(PMID:21375506). It has 3 isoforms produced by alternative splicing and the full length protein has a glycosylation site.

Publications

Species	Application	Title
human	WB	Cell Death Dis; α1,3-fucosylation of MEST promotes invasion potential of cytotrophoblast cells by activating translation initiation; Authors - Hao Wang
mouse	IHC	Nucleic Acids Res; Tissue-specific alternative polyadenylation at the imprinted gene Mest regulates allelic usage at Copg2.; Authors - Julia L MacIsaac
human	IHC	Gynecol Oncol; Increased co-expression of MEST and BRCA1 is associated with worse prognosis and immune infiltration in ovarian cancer.; Authors - Jing Zhang
human	WB	Cancer Lett; Novel target genes responsive to the anti-growth activity of triptolide in endometrial and ovarian cancer cells.; Authors - Li Haili H
human	WB,IHC	Placenta; DNA methylation-associated repression of MEST/PEG1 expression contributes to the invasion of extravillous trophoblast cells.; Authors - Wei Peng ; KD Validated
human	WB	Placenta; Maternal obesity and gestational weight gain are modestly associated with umbilical cord DNA methylation.; Authors - Keshari M Thakali