Anticorps Monoclonal anti-MEST

MEST Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1A4A1

N° de cat : 60263-1-PBS

Synonymes

1A4A1, EC:3.-.-.-, Mesoderm-specific transcript homolog protein, Paternally-expressed gene 1 protein, PEG1

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Galerie de données de validation

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  WB analysis of PC-3 using 60263-1-Ig (same clone as 60263-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 60263-1-Ig (MEST antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60263-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 60263-1-Ig (same clone as 60263-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60263-1-Ig (MEST Antibody) at dilution of 1:400 (under 10x lens). This data was developed using the same antibody clone with 60263-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 60263-1-Ig (same clone as 60263-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60263-1-Ig (MEST Antibody) at dilution of 1:400 (under 40x lens). This data was developed using the same antibody clone with 60263-1-PBS in a different storage buffer formulation.

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  IF Staining of BxPC-3 using 60263-1-Ig (same clone as 60263-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed BxPC-3 cells using 60263-1-Ig(MEST antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60263-1-PBS in a different storage buffer formulation.

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Informations sur le produit

60263-1-PBS cible MEST dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: MEST Protéine recombinante Ag11929
• Nom complet: mesoderm specific transcript homolog (mouse)
• Masse moléculaire calculée: 38 kDa
• Poids moléculaire observé: 34 kDa
• Numéro d’acquisition GenBank: BC011908
• Symbole du gène: MEST
• Identification du gène (NCBI): 4232
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MEST(Mesoderm-specific transcript homolog protein) is also named as PEG1 and belongs to the AB hydrolase superfamily. The gene is a imprinting gene, which is associated with growth of mesodermal origin cells(PMID:20339302) and plays important roles in embryo development(PMID:21375506). It is also a novel regulator of Wnt/CTNNB signalling during adipogenic differentiation(PMID:21375506). It has 3 isoforms produced by alternative splicing and the full length protein has a glycosylation site.

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