Anticorps Recombinant de lapin anti-METTL1

METTL1 Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

241108G8

N° de cat : 84272-1-RR

Synonymes

241108G8, C12orf1, EC:2.1.1.-, EC:2.1.1.33, methyltransferase like 1

---

Galerie de données de validation

• 

  WB analysis using 84272-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84272-1-RR (METTL1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of A549 using 84272-1-RR

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using METTL1 antibody (84272-1-RR, Clone: 241108G8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  FC experiment of A431 using 84272-1-RR

  1x10^6 A431 cells were intracellularly stained with 0.25 ug METTL1 Recombinant antibody (84272-1-RR, Clone:241108G8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

• 

  Affinity and Kinetic Characterization of 84272-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84272-1-RR against Human METTL1 were performed. The affinity constant is 0.526 nM.

---

Applications testées

• Résultats positifs en WB: cellules A549, cellules Caco-2, cellules HeLa, cellules HepG2, cellules HuH-7, tissu cérébral de souris
• Résultats positifs en IF/ICC: cellules A549,
• Résultats positifs en FC (Intra): cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84272-1-RR cible METTL1 dans les applications de WB, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: METTL1 Protéine recombinante Ag7055
• Nom complet: methyltransferase like 1
• Masse moléculaire calculée: 31 kDa
• Poids moléculaire observé: 31-35 kDa
• Numéro d’acquisition GenBank: BC000550
• Symbole du gène: METTL1
• Identification du gène (NCBI): 4234
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

METTL1 methyltransferase mediates m7G methylation within miRNAs and regulates cell migration via its catalytic activity. METTL1 can be inactivated by phosphorylation at Ser27 by protein kinase B (PKBα). Overexpression of METTL1 is widely observed among human cancers. It is also crucial for the regulation of chemoresistance in cancer treatment. In addition, mutations in the human N7-methylguanosine (m7G) methyltransferase complex METTL1/WDR4 cause primordial dwarfism and brain malformation.

{{ptg:RelatedPrimaryAntibodies}}