Anticorps Recombinant de lapin anti-METTL2

• Phare
• Validé par KD/KO

METTL2 Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241490A3

N° de cat : 84257-1-PBS

Synonymes

METTL2B, 241490A3, EC:2.1.1.-, methyltransferase like 2B, Methyltransferase-like protein 2B

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Galerie de données de validation

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  WB analysis of HeLa using 84257-1-RR (same clone as 84257-1-PBS)

  WB result of METTL2 antibody (84257-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-METTL2 transfected HeLa cells. This data was developed using the same antibody clone with 84257-1-PBS in a different storage buffer formulation.

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  WB analysis using 84257-1-RR (same clone as 84257-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84257-1-RR (METTL2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84257-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 84257-1-RR (same clone as 84257-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using METTL2 antibody (84257-1-RR, Clone: 241490A3 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84257-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01163-3

  Cytometric bead array standard curve of MP01163-3, METTL2B Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84257-1-PBS. Detection antibody: 84257-3-PBS. Standard: Ag10410. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 84257-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84257-1-RR against Human METTL2 were performed. The affinity constant is 0.741 nM.

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Informations sur le produit

84257-1-PBS cible METTL2 dans les applications de WB, IF/ICC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: METTL2 Protéine recombinante Ag10410
• Nom complet: methyltransferase like 2B
• Masse moléculaire calculée: 313 aa, 36 kDa
• Poids moléculaire observé: 36~43 kDa
• Numéro d’acquisition GenBank: BC107586
• Symbole du gène: METTL2B
• Identification du gène (NCBI): 55798
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

METTL2A and METTL2B are required for the threonyl and argininyl tRNA methylation. METTL2 methyltransferase is responsible for 3-methylcytosine modification of arginine tRNAs in mammals. METTL2A and METTL2B are in higher homology 99%. This antibody detects both METTL2A and METTL2B.

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