Anticorps Polyclonal de lapin anti-METTL5

• Phare
• Validé par KD/KO

METTL5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 16791-1-PBS

Synonymes

EC:2.1.1.-, HSPC133, methyltransferase like 5, Methyltransferase-like protein 5, rRNA N6-adenosine-methyltransferase METTL5

---

Galerie de données de validation

• 

  WB analysis of K-562 using 16791-1-AP (same clone as 16791-1-PBS)

  WB result of METTL5 antibody (16791-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-METTL5 transfected K-562 cells. This data was developed using the same antibody clone with 16791-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 16791-1-AP (same clone as 16791-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16791-1-AP (METTL5 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16791-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 16791-1-AP (same clone as 16791-1-PBS)

  WB result of METTL5 antibody (16791-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-METTL5 transfected HeLa cells. This data was developed using the same antibody clone with 16791-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 16791-1-AP (same clone as 16791-1-PBS)

  WB result of METTL5 antibody (16791-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-METTL5 transfected HeLa cells. This data was developed using the same antibody clone with 16791-1-PBS in a different storage buffer formulation.

• 

  IP experiment of PC-3 using 16791-1-AP (same clone as 16791-1-PBS)

  IP result of anti-METTL5 (IP:16791-1-AP, 4ug; Detection:16791-1-AP 1:500) with PC-3 cells lysate 3840 ug. This data was developed using the same antibody clone with 16791-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 16791-1-AP (same clone as 16791-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16791-1-AP (METTL5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16791-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat kidney using 16791-1-AP (same clone as 16791-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 16791-1-AP (METTL5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16791-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat kidney using 16791-1-AP (same clone as 16791-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 16791-1-AP (METTL5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16791-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 16791-1-AP (same clone as 16791-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using METTL5 antibody (16791-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 16791-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 16791-1-AP (same clone as 16791-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using METTL5 antibody (16791-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 16791-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

16791-1-PBS cible METTL5 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: METTL5 Protéine recombinante Ag10232
• Nom complet: methyltransferase like 5
• Masse moléculaire calculée: 209 aa, 24 kDa
• Poids moléculaire observé: 24-27 kDa
• Numéro d’acquisition GenBank: BC000921
• Symbole du gène: METTL5
• Identification du gène (NCBI): 29081
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

METTL5 is a member of the METTL family of S-adenosyl-methionine-dependent methyltransferases that adds m6A to a specific site, nucleotide A-1832 of 18S rRNA, hereafter referred to as m6A1832. METTL5 is localized in the nucleolus and cytoplasm.

{{ptg:RelatedPrimaryAntibodies}}