Anticorps Monoclonal anti-METTL6

METTL6 Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

1C7D4

N° de cat : 68400-1-PBS

Synonymes

1C7D4, EC:2.1.1.-, hMETTL6, methyltransferase like 6, Methyltransferase-like protein 6

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Galerie de données de validation

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  WB analysis using 68400-1-Ig (same clone as 68400-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68400-1-Ig (METTL6 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68400-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 68400-1-Ig (same clone as 68400-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 68400-1-Ig (METTL6 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68400-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 68400-1-Ig (same clone as 68400-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68400-1-Ig (METTL6 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68400-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 68400-1-Ig (same clone as 68400-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68400-1-Ig (METTL6 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68400-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 68400-1-Ig (same clone as 68400-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 68400-1-Ig (METTL6 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68400-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68400-1-PBS cible METTL6 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: METTL6 Protéine recombinante Ag9714
• Nom complet: methyltransferase like 6
• Masse moléculaire calculée: 255 aa, 30 kDa
• Poids moléculaire observé: 30-32 kDa
• Numéro d’acquisition GenBank: BC022400
• Symbole du gène: METTL6
• Identification du gène (NCBI): 131965
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Methyltransferase-like protein 6 (METTL6) belongs to the METL family. METTL6 is as a crucial regulator of tumor cell growth, and METTL6 is a bona fide transfer RNA (tRNA) methyltransferase, catalyzing the formation of 3-methylcytidine at C32 of specific serine tRNA isoacceptors (PMID:32923617). METTL6 is a member of the RNA methyltransferase family that has been identified in many cancers. The mRNA expression levels of METTL6 were significantly upregulated in HCC tumor tissues compared to that in adjacent non tumor tissues. CRISPR/Cas9 mediated knockout of METTL6 in hepatocellular carcinoma cell lines remarkably inhibited colony formation, cell proliferation, cell migration, cell invasion and cell attachment ability (PMID:34913069).

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