Anticorps Recombinant de lapin anti-METTL6

METTL6 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

241482F12

N° de cat : 84209-4-RR

Synonymes

tRNA N(3)-methylcytidine methyltransferase METTL6, Methyltransferase-like protein 6, methyltransferase like 6, hMETTL6, EC:2.1.1.-

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Galerie de données de validation

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  WB analysis using 84209-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84209-4-RR (METTL6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84209-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84209-4-RR against Human METTL6 were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules HepG2, cellules Jurkat, cellules K-562, cellules LNCaP

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84209-4-RR cible METTL6 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: METTL6 Protéine recombinante Ag9714
• Nom complet: methyltransferase like 6
• Masse moléculaire calculée: 255 aa, 30 kDa
• Poids moléculaire observé: 30-32 kDa
• Numéro d’acquisition GenBank: BC022400
• Symbole du gène: METTL6
• Identification du gène (NCBI): 131965
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Methyltransferase-like protein 6 (METTL6) belongs to the METL family. METTL6 is as a crucial regulator of tumor cell growth, and METTL6 is a bona fide transfer RNA (tRNA) methyltransferase, catalyzing the formation of 3-methylcytidine at C32 of specific serine tRNA isoacceptors (PMID:32923617). METTL6 is a member of the RNA methyltransferase family that has been identified in many cancers. The mRNA expression levels of METTL6 were significantly upregulated in HCC tumor tissues compared to that in adjacent non-tumor tissues. CRISPR/Cas9 mediated knockout of METTL6 in hepatocellular carcinoma cell lines remarkably inhibited colony formation, cell proliferation, cell migration, cell invasion and cell attachment ability (PMID:34913069).

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