Anticorps Recombinant de lapin anti-METTL6

METTL6 Recombinant Antibody for IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241482F9

N° de cat : 84209-5-PBS

Synonymes

241482F9, EC:2.1.1.-, hMETTL6, methyltransferase like 6, Methyltransferase-like protein 6

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Galerie de données de validation

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  IHC staining of mouse testis using 84209-5-RR (same clone as 84209-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84209-5-RR (METTL6 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84209-5-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84209-5-RR (same clone as 84209-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using METTL6 antibody (84209-5-RR, Clone: 241482F9 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84209-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84209-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84209-5-RR against Human METTL6 were performed. The affinity constant is 7.69 nM.

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Informations sur le produit

84209-5-PBS cible METTL6 dans les applications de IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: METTL6 Protéine recombinante Ag9714
• Nom complet: methyltransferase like 6
• Masse moléculaire calculée: 255 aa, 30 kDa
• Numéro d’acquisition GenBank: BC022400
• Symbole du gène: METTL6
• Identification du gène (NCBI): 131965
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Methyltransferase-like protein 6 (METTL6) belongs to the METL family. METTL6 is as a crucial regulator of tumor cell growth, and METTL6 is a bona fide transfer RNA (tRNA) methyltransferase, catalyzing the formation of 3-methylcytidine at C32 of specific serine tRNA isoacceptors (PMID:32923617). METTL6 is a member of the RNA methyltransferase family that has been identified in many cancers. The mRNA expression levels of METTL6 were significantly upregulated in HCC tumor tissues compared to that in adjacent non tumor tissues. CRISPR/Cas9 mediated knockout of METTL6 in hepatocellular carcinoma cell lines remarkably inhibited colony formation, cell proliferation, cell migration, cell invasion and cell attachment ability (PMID:34913069).

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