Anticorps Polyclonal de lapin anti-MFAP4

MFAP4 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(9)

N° de cat : 17661-1-AP

Synonymes

Microfibril-associated glycoprotein 4

---

Galerie de données de validation

• 

  WB analysis of human kidney using 17661-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 17661-1-AP (MFAP4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat lung using 17661-1-AP

  rat lung tissue were subjected to SDS PAGE followed by western blot with 17661-1-AP (MFAP4 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

• 

  WB analysis of mouse heart using 17661-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 17661-1-AP (MFAP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 17661-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 17661-1-AP (MFAP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung using 17661-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 17661-1-AP (MFAP4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 17661-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17661-1-AP (MFAP4 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 17661-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17661-1-AP (MFAP4 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 17661-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17661-1-AP (MFAP4 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 17661-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17661-1-AP (MFAP4 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human skin using 17661-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 17661-1-AP (MFAP4 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human skin using 17661-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 17661-1-AP (MFAP4 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 17661-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17661-1-AP (MFAP4 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 17661-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17661-1-AP (MFAP4 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 17661-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17661-1-AP (MFAP4 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 17661-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17661-1-AP (MFAP4 antibody) at dilution of 1:100 (under 40x lens).

---

Applications testées

• Résultats positifs en WB: tissu rénal humain, tissu cardiaque de souris, tissu pulmonaire de rat, tissu pulmonaire de souris
• Résultats positifs en IHC: tissu pulmonaire humain, tissu cutané humain, tissu placentaire humain, tissu splénique humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 8 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Informations sur le produit

17661-1-AP cible MFAP4 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MFAP4 Protéine recombinante Ag11920
• Nom complet: microfibrillar-associated protein 4
• Masse moléculaire calculée: 255 aa, 29 kDa
• Poids moléculaire observé: 36 kDa
• Numéro d’acquisition GenBank: BC062415
• Symbole du gène: MFAP4
• Identification du gène (NCBI): 4239
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Microfibrillar-associated protein 4 (MFAP4) is a extracellular matrix glycoprotein containing a fibrinogen C-terminal domain and a N-terminal located integrin-binding motif. It could be involved in calcium-dependent cell adhesion or intercellular interactions. MFAP4 gene is commonly deleted in Smith-Magenis syndrome patients. (PMID: 7633408; 16867155; 10542261; 18322703)

Publications

Species	Application	Title
human	WB,IHC	Thorax; A large lung gene expression study identifying fibulin-5 as a novel player in tissue repair in COPD.; Authors - Corry-Anke Brandsma
human	WB	Front Pharmacol; MFAP4 is a novel prognostic biomarker in glioma correlating with immunotherapy resistance and ferroptosis; Authors - Yuanhao Lv
human	WB	J Proteome Res; Strategy for SRM-based verification of biomarker candidates discovered by iTRAQ method in limited breast cancer tissue samples.; Authors - Muraoka Satoshi S
human	IHC,IF	FASEB J; The "Matrisome" reveals the characterization of skin keloid microenvironment.; Authors - Shikun Zhang
mouse	WB	J Proteomics; FOXL1 + Telocytes in mouse colon orchestrate extracellular matrix biodynamics and wound repair resolution; Authors - Veronique Pomerleau
mouse	WB,IF	BMC Biol; The m6A writer KIAA1429 regulates photoaging progression via MFAP4-dependent collagen synthesis; Authors - Yuanyuan Liu

{{ptg:RelatedPrimaryAntibodies}}