Anticorps Monoclonal anti-MFF

MFF Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

2

CloneNo.

1G4B12

Avis (1)

N° de cat : 66527-1-Ig

Synonymes

AD033, C2orf33, MFF, mitochondrial fission factor

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Galerie de données de validation

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  WB analysis of pig brain using 66527-1-Ig

  pig/rat/mouse brain tissues were subjected to SDS PAGE followed by western blot with 66527-1-Ig (MFF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 66527-1-Ig

  L02 cells were subjected to SDS PAGE followed by western blot with 66527-1-Ig (MFF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 66527-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66527-1-Ig (MFF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 66527-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66527-1-Ig (MFF antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 66527-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66527-1-Ig (MFF antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 66527-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using MFF antibody (66527-1-Ig, Clone: 1G4B12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de porc, cellules HepG2, cellules L02, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu cérébral de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

66527-1-Ig cible MFF dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: MFF Protéine recombinante Ag9990
• Nom complet: mitochondrial fission factor
• Masse moléculaire calculée: 38 kDa
• Poids moléculaire observé: 35-38 kDa
• Numéro d’acquisition GenBank: BC000797
• Symbole du gène: MFF
• Identification du gène (NCBI): 56947
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MFF (mitochondrial fission factor) is a mitochondrial outer membrane protein that is involved in mitochondrial localization of Drp1 and mitochondrial fission. Multiple isoforms of MFF exist due to the alternative splicing. This antibody recognizes the endogenous MFF protein around 26-29 kDa and 35-38 kDa.

Publications

Species	Application	Title
rat	WB	Phytother Res; Shenlian extract decreases mitochondrial autophagy to regulate mitochondrial function in microvascular to alleviate coronary artery no-reflow; Authors - Jing-Jing Li
mouse	WB	J Adv Res; Ceramide-1-phosphate alleviates high-altitude pulmonary edema by stabilizing circadian ARNTL-mediated mitochondrial dynamics; Authors - Liuyang Tian

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

JAE HO (Verified Customer) (05-06-2019)

25 ug of PC3 cell lysates were used for western blot.500 ug of cell lysates and 2.5 ug of antibodies were used for IP.Comparing to "17090-1-AP", it looks less titer but it works well for WB and IP.

• Applications: Western Blot, Immunoprecipitation,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: PC3 cell