Anticorps Monoclonal anti-MFN1

MFN1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

3F11C11

N° de cat : 66776-1-PBS

Synonymes

3F11C11, EC:3.6.5.-, Fzo homolog, hfzo1, hfzo2

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Galerie de données de validation

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  WB analysis using 66776-1-Ig (same clone as 66776-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66776-1-Ig (MFN1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66776-1-PBS in a different storage buffer formulation.

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  WB analysis using 66776-1-Ig (same clone as 66776-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66776-1-Ig (MFN1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66776-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66776-1-Ig (same clone as 66776-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66776-1-Ig (MFN1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66776-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66776-1-Ig (same clone as 66776-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66776-1-Ig (MFN1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66776-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66776-1-Ig (same clone as 66776-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66776-1-Ig (MFN1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66776-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66776-1-Ig (same clone as 66776-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66776-1-Ig (MFN1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66776-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66776-1-Ig (same clone as 66776-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MFN1 antibody (66776-1-Ig, Clone: 3F11C11 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66776-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66776-1-PBS cible MFN1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: MFN1 Protéine recombinante Ag4890
• Nom complet: mitofusin 1
• Masse moléculaire calculée: 741 aa, 84 kDa
• Poids moléculaire observé: 84 kDa
• Numéro d’acquisition GenBank: BC040557
• Symbole du gène: MFN1
• Identification du gène (NCBI): 55669
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Mitofusin-1 (MFN1) is a mediator of mitochondrial fusion. This protein and mitofusin 2 are homologs of the Drosophila protein fuzzy onion (Fzo). Mitofusins are large predicted GTPases located in outer mitochondrial membrane. They are essential for outer membrane fusion by interacting with each other to facilitate mitochondrial targeting. The mitofusins are the first known protein mediator of mitochondrial fusion, and mediate developmentally regulated post-meiotic fusion of mitochondria. Mitofusin 1 and mitofusin 2 are ubiquitinated in a PINK1/parkin-dependent manner upon induction of mitophagy(PMID: 20871098).

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