Anticorps Polyclonal de lapin anti-MFN2

• Phare
• Validé par KD/KO

MFN2 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (7)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(461)

Avis (4)

N° de cat : 12186-1-AP

Synonymes

CPRP1, EC:3.6.5.-, HSG, KIAA0214, MARF

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Galerie de données de validation

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  WB analysis using 12186-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12186-1-AP (MFN2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 12186-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12186-1-AP (MFN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12186-1-AP

  WB result of MFN2 antibody (12186-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MFN2 transfected HeLa cells.

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  WB analysis using 12186-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12186-1-AP (MFN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 12186-1-AP

  IP result of anti-MFN2 (IP:12186-1-AP, 4ug; Detection:12186-1-AP 1:10000) with mouse kidney tissue lysate 2160 ug.

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  IHC staining of human colon cancer using 12186-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12186-1-AP (MFN2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 12186-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12186-1-AP (MFN2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 12186-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12186-1-AP (MFN2 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules HeLa, tissu cardiaque de rat, tissu cérébral de rat, tissu hépatique de rat, tissu hépatique de souris, tissu rénal de rat, tissu rénal de souris
• Résultats positifs en IP: tissu rénal de souris,
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu rénal de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 18 publications below
• WB: See 438 publications below
• IHC: See 29 publications below
• IF: See 39 publications below
• IP: See 8 publications below
• CoIP: See 4 publications below

Informations sur le produit

12186-1-AP cible MFN2 dans les applications de WB, IHC, IF, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, Chèvre, Humain, poisson-zèbre, porc, poulet, singe, souris, duck
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MFN2 Protéine recombinante Ag2845
• Nom complet: mitofusin 2
• Masse moléculaire calculée: 757 aa, 86 kDa
• Poids moléculaire observé: 86 kDa
• Numéro d’acquisition GenBank: BC017061
• Symbole du gène: MFN2
• Identification du gène (NCBI): 9927
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MFN2, also named as CPRP1 and KIAA0214, belongs to the mitofusin family. It is an Essential transmembrane GTPase, which mediates mitochondrial fusion. MFN2 acts independently of the cytoskeleton. It therefore plays a central role in mitochondrial metabolism and may be associated with obesity and/or apoptosis processes. Overexpression of MFN2 induces the formation of mitochondrial networks. It plays an important role in the regulation of vascular smooth muscle cell proliferation. Defects in MFN2 are the cause of Charcot-Marie-Tooth disease type 2A2 (CMT2A2). Defects in MFN2 are the cause of Charcot-Marie-Tooth disease type 6 (CMT6). Ubiquitinated forms of Mfn2 (mono- and polyubiquitinated) are present during mitophagy.

Publications

Species	Application	Title
mouse	WB	Nature; Cellular ATP demand creates metabolically distinct subpopulations of mitochondria; Authors - Keun Woo Ryu ; KO Validated
mouse	WB	Cell; Mitocytosis, a migrasome-mediated mitochondrial quality-control process.; Authors - Haifeng Jiao ; KD Validated
mouse	WB	Cell Metab; Mitochondrial Dynamics Is Critical for the Full Pluripotency and Embryonic Developmental Potential of Pluripotent Stem Cells.; Authors - Xiuying Zhong
mouse	WB	ACS Cent Sci; Macrophage Inactivation by Small Molecule Wedelolactone via Targeting sEH for the Treatment of LPS-Induced Acute Lung Injury; Authors - Juan Zhang
mouse,human	WB,IF	Nat Commun; Engineered hypoxia-responsive albumin nanoparticles mediating mitophagy regulation for cancer therapy; Authors - Wenyan Wang
mouse	WB	Mol Cell; Serine synthesis sustains macrophage IL-1β production via NAD+-dependent protein acetylation; Authors - Chuanlong Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

S (Verified Customer) (09-23-2024)

Good

• Applications: Western Blot
• Primary Antibody Dilution: 1000
• Cell Tissue Type: A549

MFN2 Antibody Western Blot validation (1000 dilution) in A549 (Cat no:12186-1-AP)

FH

Nin (Verified Customer) (02-27-2023)

A reliable Ab to detect endogenous Mfn2

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: mouse brain lysate

MFN2 Antibody Western Blot validation (1:3000 dilution) in mouse brain lysate (Cat no:12186-1-AP)

FH

Mi (Verified Customer) (02-21-2023)

This antibody works pretty well in adipocytes, we got a single and clean band at the expected size.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: adipocytes

FH

Julia (Verified Customer) (11-16-2018)

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Hek293 cells

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