Anticorps Monoclonal anti-MFN2

• Phare
• Validé par KD/KO

MFN2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

5F3B3

N° de cat : 67487-1-PBS

Synonymes

5F3B3, CPRP1, EC:3.6.5.-, HSG, KIAA0214

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Galerie de données de validation

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  WB analysis of HeLa using 67487-1-Ig (same clone as 67487-1-PBS)

  WB result of MFN2 antibody (67487-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MFN2 transfected HeLa cells. This data was developed using the same antibody clone with 67487-1-PBS in a different storage buffer formulation.

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  WB analysis using 67487-1-Ig (same clone as 67487-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67487-1-Ig (MFN2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67487-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 67487-1-Ig (same clone as 67487-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67487-1-Ig (MFN2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67487-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 67487-1-Ig (same clone as 67487-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67487-1-Ig (MFN2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67487-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67487-1-Ig (same clone as 67487-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MFN2 antibody (67487-1-Ig, Clone: 5F3B3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67487-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67487-1-PBS cible MFN2 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: MFN2 Protéine recombinante Ag29873
• Nom complet: mitofusin 2
• Masse moléculaire calculée: 757 aa, 86 kDa
• Poids moléculaire observé: 86 kDa
• Numéro d’acquisition GenBank: BC017061
• Symbole du gène: MFN2
• Identification du gène (NCBI): 9927
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MFN2, also named as CPRP1 and KIAA0214, belongs to the mitofusin family. It is an Essential transmembrane GTPase, which mediates mitochondrial fusion. MFN2 acts independently of the cytoskeleton. It therefore plays a central role in mitochondrial metabolism and may be associated with obesity and/or apoptosis processes. Overexpression of MFN2 induces the formation of mitochondrial networks. It plays an important role in the regulation of vascular smooth muscle cell proliferation. Defects in MFN2 are the cause of Charcot-Marie-Tooth disease type 2A2 (CMT2A2). Defects in MFN2 are the cause of Charcot-Marie-Tooth disease type 6 (CMT6). Ubiquitinated forms of Mfn2 (mono- and polyubiquitinated) are present during mitophagy.

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