Anticorps Polyclonal de lapin anti-MGAT1

MGAT1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(10)

N° de cat : 15103-1-AP

Synonymes

GlcNAc-T I, GLCNAC TI, GlcNAc T I, GGNT1, EC:2.4.1.101

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Galerie de données de validation

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  WB analysis of Y79 using 15103-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 15103-1-AP (MGAT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 15103-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15103-1-AP (MGAT1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 15103-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15103-1-AP (MGAT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 15103-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15103-1-AP (MGAT1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of mouse liver using 15103-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15103-1-AP (MGAT1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of mouse heart using 15103-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15103-1-AP (MGAT1 Antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of mouse heart using 15103-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15103-1-AP (MGAT1 Antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human heart using 15103-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15103-1-AP (MGAT1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 15103-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15103-1-AP (MGAT1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 15103-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using MGAT1 antibody (15103-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules Y79, cellules HepG2
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu cardiaque de souris, tissu cardiaque humain, tissu hépatique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 9 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

15103-1-AP cible MGAT1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MGAT1 Protéine recombinante Ag7446
• Nom complet: mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase
• Masse moléculaire calculée: 39 kDa
• Poids moléculaire observé: 51-55 kDa
• Numéro d’acquisition GenBank: BC003575
• Symbole du gène: MGAT1
• Identification du gène (NCBI): 4245
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB	Arthritis Rheum; Alterations of high-mannose type N-glycosylation in human and mouse osteoarthritis cartilage.; Authors - Urita Atsushi A
	WB	Cell Mol Life Sci; N-terminome analyses underscore the prevalence of SPPL3-mediated intramembrane proteolysis among Golgi-resident enzymes and its role in Golgi enzyme secretion.; Authors - Laura Hobohm
mouse	WB	FASEB J; Reduced myocyte complex N-glycosylation causes dilated cardiomyopathy.; Authors - Andrew R Ednie
human, rat	WB	Schizophr Res; Abnormal N-acetylglucosaminyltransferase expression in prefrontal cortex in schizophrenia.; Authors - Jordyn M Kippe
mouse	WB	Liver Transpl; Monoacylglycerol Acyltransferase 1 Knockdown Exacerbates Hepatic Ischemia/Reperfusion Injury in Mice With Hepatic Steatosis.; Authors - Kim H H Liss
rat	WB,IF	Sci Rep; Bupivacaine reduces GlyT1 expression by potentiating the p-AMPKα/BDNF signalling pathway in spinal astrocytes of rats.; Authors - Kaimei Lu

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