Anticorps Polyclonal de lapin anti-MGAT3

• Phare
• Validé par KD/KO

MGAT3 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 17869-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of COLO 320 using 17869-1-AP (same clone as 17869-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 17869-1-AP (MGAT3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17869-1-PBS in a different storage buffer formulation.

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  WB analysis of Caco-2 using 17869-1-AP (same clone as 17869-1-PBS)

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 17869-1-AP (MGAT3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17869-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 17869-1-AP (same clone as 17869-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17869-1-AP (MGAT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17869-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 17869-1-AP (same clone as 17869-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17869-1-AP (MGAT3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17869-1-PBS in a different storage buffer formulation.

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Informations sur le produit

17869-1-PBS cible MGAT3 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MGAT3 Protéine recombinante Ag12352
• Nom complet: mannosyl (beta-1,4-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase
• Masse moléculaire calculée: 533 aa, 61 kDa
• Poids moléculaire observé: 61 kDa
• Numéro d’acquisition GenBank: BC075026
• Symbole du gène: MGAT3
• Identification du gène (NCBI): 4248
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

In the small intestine, ~75% of triacylglycerol (TG) synthesis occurs via the monoacylglycerol acyltransferase (MGAT) pathway.Three MGAT isoforms (MGAT1, MGAT2 and MGAT3) have been identifed based on their extensive homology to DGAT2. MGAT1 is primarily expressed in the adipose tissue, stomach and kidney but not in the small intestine. MGAT2 and MGAT3 are most highly expressed in the small intestine, suggesting that these two MGAT forms are responsible for the resynthesis of dietary TG that is packaged into chylomicrons.

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