Anticorps Polyclonal de lapin anti-MGAT5B

MGAT5B Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 16993-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of HepG2 using 16993-1-AP (same clone as 16993-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16993-1-AP (MGAT5B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16993-1-PBS in a different storage buffer formulation.

• 

  WB analysis of SH-SY5Y using 16993-1-AP (same clone as 16993-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 16993-1-AP (MGAT5B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16993-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 16993-1-AP (same clone as 16993-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 16993-1-AP (MGAT5B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16993-1-PBS in a different storage buffer formulation.

• 

  WB analysis of C6 using 16993-1-AP (same clone as 16993-1-PBS)

  C6 cells were subjected to SDS PAGE followed by western blot with 16993-1-AP (MGAT5B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16993-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 16993-1-AP (same clone as 16993-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16993-1-AP (MGAT5B antibody) at dilution of 1:1600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16993-1-PBS in a different storage buffer formulation.

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  IP experiment of Jurkat using 16993-1-AP (same clone as 16993-1-PBS)

  IP result of anti-MGAT5B (IP:16993-1-AP, 4ug; Detection:16993-1-AP 1:700) with Jurkat cells lysate 3600ug. This data was developed using the same antibody clone with 16993-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 16993-1-AP (same clone as 16993-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16993-1-AP (MGAT5B antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16993-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 16993-1-AP (same clone as 16993-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16993-1-AP (MGAT5B antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16993-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 16993-1-AP (same clone as 16993-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16993-1-AP (MGAT5B antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16993-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 16993-1-AP (same clone as 16993-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16993-1-AP (MGAT5B antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16993-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 16993-1-AP (same clone as 16993-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 16993-1-AP (MGAT5B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16993-1-PBS in a different storage buffer formulation.

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Informations sur le produit

16993-1-PBS cible MGAT5B dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MGAT5B Protéine recombinante Ag10678
• Nom complet: mannosyl (alpha-1,6-)-glycoprotein beta-1,6-N-acetyl-glucosaminyltransferase, isozyme B
• Masse moléculaire calculée: 394 aa, 44 kDa, 89 kDa
• Poids moléculaire observé: 89-100 kDa
• Numéro d’acquisition GenBank: BC062354
• Symbole du gène: MGAT5B
• Identification du gène (NCBI): 146664
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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