Anticorps Recombinant de lapin anti-MGEA5

MGEA5 Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

242263A6

N° de cat : 84722-4-RR

Synonymes

OGA, HEXC, EC:3.2.1.169, Beta-N-acetylhexosaminidase, Beta-N-acetylglucosaminidase

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Galerie de données de validation

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  WB analysis using 84722-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84722-4-RR (MGEA5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human rectal cancer using 84722-4-RR

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 84722-4-RR (MGEA5 antibody) at dilution of 1:250 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 84722-4-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MGEA5 antibody (84722-4-RR, Clone: 242263A6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 84722-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84722-4-RR against Human MGEA5 were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules JAR
• Résultats positifs en IHC: human rectal cancer tissue, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:125-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84722-4-RR cible MGEA5 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: MGEA5 Protéine recombinante Ag6405
• Nom complet: meningioma expressed antigen 5 (hyaluronidase)
• Masse moléculaire calculée: 103 kDa
• Poids moléculaire observé: 130-140 kDa
• Numéro d’acquisition GenBank: BC039583
• Symbole du gène: MGEA5
• Identification du gène (NCBI): 10724
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Human meningioma-expressed antigen 5 (MGEA5) has two putative domains including protein O-GlcNAcase domain and histone acetyltransferase domain, therefore it is often called bifunctional protein NCOAT. Three isoforms of MEGE5 are produced by alternative splicing. MGEA5 was found to be regulated to reduce the state of glycosylation of transcitpional activators while increasing the acetylation of histones to allow for the concerted activation of eukaryotic gene transcription, for instance, acetylation of Lys8 of histone H4 and Lys 14 of histone H3 are resulted from acetyltransferase activity. In addition, single nucleotide polymorphism in MGE5A is associated with type 2 diabetes in Mexican Americans. Two bands at 130kDa and 75 kDa could be detected using the present rabbit polyclonal antibody 14711-1-AP, which is consistent with results in a related reference (PubMed:11341771).

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