Anticorps Polyclonal de lapin anti-MGLL

• Phare
• Validé par KD/KO

MGLL Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

N° de cat : 14986-1-PBS

Synonymes

EC:3.1.1.23, HU K5, HUK5, HU-K5, Monoacylglycerol lipase

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Galerie de données de validation

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  WB analysis of HepG2 using 14986-1-AP (same clone as 14986-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14986-1-AP (MGLL antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14986-1-PBS in a different storage buffer formulation.

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  WB analysis using 14986-1-AP (same clone as 14986-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14986-1-AP (MGLL antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14986-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse liver using 14986-1-AP (same clone as 14986-1-PBS)

  IP result of anti-MGLL (IP:14986-1-AP, 4ug; Detection:14986-1-AP 1:300) with mouse liver tissue lysate 4000ug. This data was developed using the same antibody clone with 14986-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 14986-1-PBS

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14986-1-AP (MGLL antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 14986-1-PBS

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14986-1-AP (MGLL antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 14986-1-AP (same clone as 14986-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14986-1-AP (MGLL antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14986-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 14986-1-AP (same clone as 14986-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14986-1-AP (MGLL antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14986-1-PBS in a different storage buffer formulation.

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Informations sur le produit

14986-1-PBS cible MGLL dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MGLL Protéine recombinante Ag6970
• Nom complet: monoglyceride lipase
• Masse moléculaire calculée: 33 kDa
• Poids moléculaire observé: 33 kDa
• Numéro d’acquisition GenBank: BC000551
• Symbole du gène: MGLL
• Identification du gène (NCBI): 11343
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MGLL (monoglyceride lipase) is also named as MAGL, HU-K5 and belongs to the monoacylglycerol lipase family. It converts monoacylglycerides to free fatty acids and glycerol and functions together with hormone-sensitive lipase (HSL) in the mobilization of fatty acids from the triglyceride stores of adipocytes. MGL protein is ubiquitously expressed in kidney, spleen, heart, liver, stomach, brain, lung, adrenal gland, adipose tissue and in brain,there are two bands of 33 kDa and 35 kDa can be detected. The single protein species expressed in muscle is larger (around 40 kDa) (PMID:11470505). This protein can exsit as a homodimer (PMID:19957260).

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