Anticorps Monoclonal anti-MGME1

• Phare
• Validé par KD/KO

MGME1 Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

Publications(2)

CloneNo.

2C4G1

N° de cat : 67468-1-Ig

Synonymes

C20orf72, 2C4G1, DDK1, EC:3.1.-.-, Mitochondrial genome maintenance exonuclease 1

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Galerie de données de validation

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  WB analysis using 67468-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67468-1-Ig (MGME1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 67468-1-Ig

  WB result of MGME1 antibody (67468-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MGME1 transfected HeLa cells.

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  WB analysis of HL-60 using 67468-1-Ig

  HL-60 cells were subjected to SDS PAGE followed by western blot with 67468-1-Ig (MGME1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 67468-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67468-1-Ig (MGME1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 67468-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67468-1-Ig (MGME1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 67468-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67468-1-Ig (MGME1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 67468-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67468-1-Ig (MGME1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 67468-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67468-1-Ig (MGME1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 67468-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67468-1-Ig (MGME1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A549, cellules HepG2, cellules HL-60, cellules Jurkat, cellules K-562, cellules LNCaP
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:150-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

67468-1-Ig cible MGME1 dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: MGME1 Protéine recombinante Ag19644
• Nom complet: chromosome 20 open reading frame 72
• Masse moléculaire calculée: 344 aa, 39 kDa
• Poids moléculaire observé: 36-39 kDa
• Numéro d’acquisition GenBank: BC016869
• Symbole du gène: MGME1
• Identification du gène (NCBI): 92667
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Aging (Albany NY); MGME1 associates with poor prognosis and is vital for cell proliferation in lower-grade glioma; Authors - Feng Xiao
human	WB,IP	Biochem Biophys Res Commun; Unspliced XBP1 enhences metabolic reprogramming in colorectal cancer cells by interfering with the mitochondrial localization of MGME1; Authors - Jiandong Zhang

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