Anticorps Recombinant de lapin anti-MGMT

MGMT Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

243130G10

N° de cat : 86115-5-PBS

Synonymes

6-O-methylguanine-DNA methyltransferase, EC:2.1.1.63, Methylated-DNA--protein-cysteine methyltransferase, O-6-methylguanine-DNA-alkyltransferase

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Galerie de données de validation

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  WB analysis using 86115-5-RR (same clone as 86115-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 89933-4-RR (MGMT antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86115-5-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 86115-5-RR (same clone as 86115-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 86115-5-RR (MGMT antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86115-5-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 86115-5-RR (same clone as 86115-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 86115-5-RR (MGMT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86115-5-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 86115-5-RR (same clone as 86115-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using MGMT antibody (89933-4-RR, Clone: 243130G10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86115-5-PBS in a different storage buffer formulation.

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Informations sur le produit

86115-5-PBS cible MGMT dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: MGMT Protéine recombinante Ag9996
• Nom complet: O-6-methylguanine-DNA methyltransferase
• Masse moléculaire calculée: 22 kDa
• Poids moléculaire observé: 22 kDa
• Numéro d’acquisition GenBank: BC000824
• Symbole du gène: MGMT
• Identification du gène (NCBI): 4255
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MGMT is the primary vehicle for cellular removal of alkyl lesions from the O-6 position of guanine and the O-4 position of thymine. While key to the maintenance of genomic integrity, MGMT also removes damage induced by alkylating chemotherapies, inhibiting the efficacy of cancer treatment (PMID:23065697). MGMT is the primary mechanism for the removal of alkylation damage from the O-6 position of guanine (PMID: 17482892). The O-6 position of guanine is one of several positions in DNA bases to which alkyl groups are attached in SN1 alkylation reactions, and this repair has been well-characterized in mammalian cells and via MGMT homologs in bacteria and Archaea (PMID: 10767620).

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