Anticorps Recombinant de lapin anti-MHC Class II (I-A/I-E)

MHC Class II (I-A/I-E) Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

FC

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

M5/114.15.2

N° de cat : CL488-FcA65663

Synonymes

H2Aa, Aalpha, H 2Aa, H2-Aa, Ia 1

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Galerie de données de validation

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  FC experiment of mouse splenocytes using CL488-FcA65663

  1x10^6 mouse splenocytes were surface stained with FcZero-rAb™ PE Anti-Mouse CD3e (145-2C11), and 0.25 ug CoraLite® Plus 488 Anti-Mouse MHC Class II (I-A/I-E) (M5/114.15.2) Rabbit IgG RecAb (CL488-FcA65663, Clone: M5/114.15.2) or FcZero-rAb™ CoraLite® Plus 488 Rabbit IgG Isotype Control Recombinant Antibody (CL488-FcA98136, Clone: 240953C9). Cells were not fixed.

• 

  FC experiment of mouse splenocytes using CL488-FcA65663

  1x10^6 mouse splenocytes were surface stained with 0.25 ug CoraLite® Plus 488 Anti-Mouse MHC Class II (I-A/I-E) (M5/114.15.2) Rabbit IgG RecAb (CL488-FcA65663, Clone: M5/114.15.2) (red) or FcZero-rAb™ CoraLite® Plus 488 Rabbit IgG Isotype Control Recombinant Antibody (CL488-FcA98136, Clone: 240953C9) (blue). Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: mouse splenocytes,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-FcA65663 cible MHC Class II (I-A/I-E) dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: s/o
• Nom complet: histocompatibility 2, class II antigen A, alpha
• Numéro d’acquisition GenBank: BC031711
• Symbole du gène: MHC Class II
• Identification du gène (NCBI): 14960
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Major histocompatibility complex (MHC) class II molecules are heterodimeric transmembrane glycoproteins that are expressed on the surface of antigen-presenting cells such as dendritic cells, macrophages, and B cells. The M5/114.15.2 antibody detects polymorphic determinants present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2b, H-2d, H-2q, H-2p, H-2r and H-2u haplotypes but not from mice carrying the H-2s or H-2f haplotypes (PMID: 6170707). This antibody recognizes both I-A and I-E subregion-encoded Ia glycoproteins (I-Ab, I-Ad, I-Aq, I-Ed, I-Ek, not I-Af, I-Ak, or I-As). M5/114 antibody is reported to inhibit I-A subregion-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not the H-2f, H-2k, H-2s haplotypes (PMID: 6173436).

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